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ipo9 pab0154 antibody  (Abnova)

 
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    Structured Review

    Abnova ipo9 pab0154 antibody
    Ipo9 Pab0154 Antibody, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ipo9+pab0154+antibody/ipo9+antibody/pm36122768-213-16-20
    Average 90 stars, based on 1 article reviews
    ipo9 pab0154 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    SDS Page:

    Article Title: Regulation of nuclear actin levels and MRTF/SRF target gene expression during PC6.3 cell differentiation.
    Article Snippet: SRF (5147, 1:750), MRTF-A (14760S, 1:1000) and cofilin (3312, 1:1000) antibodies were from Cell Signaling Technology, Ipo9 (PAB0154, 1:1000) from Abnova and MRTF-A G8 (sc-390 324, 1:50) from Santa Cruz Biotechnology.

    Western Blot:

    Article Title: Regulation of nuclear actin levels and MRTF/SRF target gene expression during PC6.3 cell differentiation.
    Article Snippet: SRF (5147, 1:750), MRTF-A (14760S, 1:1000) and cofilin (3312, 1:1000) antibodies were from Cell Signaling Technology, Ipo9 (PAB0154, 1:1000) from Abnova and MRTF-A G8 (sc-390 324, 1:50) from Santa Cruz Biotechnology.



    Similar Products

    ipo9 pab0154 antibody  (Abnova)
    90
    Abnova ipo9 pab0154 antibody
    Ipo9 Pab0154 Antibody, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ipo9+pab0154+antibody/ipo9+antibody/pm36122768-213-16-20
    Average 90 stars, based on 1 article reviews
    ipo9 pab0154 antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    antibody ipo9 pab0154  (Abnova)
    90
      Buy from Supplier

    Image Search Results


    Actin associates with RNA splicing factors and the disturbance of nuclear actin dynamics affects alternative mRNA splicing of different minigenes. (A) Confocal microscopy images of a BiFC assay between different splicing factors and actin. Tetracycline-induced HA–GFP1-10–actin U2OS cells transiently transfected with the indicated Flag–GFP11 constructs. Data shown as in <xref ref-type=Fig. 4 B. The right-hand column is a magnified image of CDC5L from the indicated area. Scale bar: 20 μm. (B) Schematic of the minigene assay. The white boxes represent exons and the black lines between the boxes represent introns. Arrows indicate the position of the primers used in the assay. (C) RT-PCR analysis of different minigene splicing from control, Ipo9 and Exp6 siRNA-treated NIH3T3 cells. The order of the used minigenes (SMN1, SMN2, RPGR wt, RPGR −2C and RPGR −6C) is indicated in the figure. The minigene mRNA products are indicated on the left. Quantification from three independent experiments as mean±s.d. percentage of exon inclusion is shown underneath the images. Significant differences between indicated samples are marked with asterisks: SMN2: control siRNA and Ipo9 siRNA ( P =0.03), RPGR −2C: control siRNA and Ipo9 siRNA ( P =0.02) and control siRNA and Exp6 siRNA ( P =0.04). " width="100%" height="100%">

    Journal: Journal of Cell Science

    Article Title: Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing

    doi: 10.1242/jcs.226852

    Figure Lengend Snippet: Actin associates with RNA splicing factors and the disturbance of nuclear actin dynamics affects alternative mRNA splicing of different minigenes. (A) Confocal microscopy images of a BiFC assay between different splicing factors and actin. Tetracycline-induced HA–GFP1-10–actin U2OS cells transiently transfected with the indicated Flag–GFP11 constructs. Data shown as in Fig. 4 B. The right-hand column is a magnified image of CDC5L from the indicated area. Scale bar: 20 μm. (B) Schematic of the minigene assay. The white boxes represent exons and the black lines between the boxes represent introns. Arrows indicate the position of the primers used in the assay. (C) RT-PCR analysis of different minigene splicing from control, Ipo9 and Exp6 siRNA-treated NIH3T3 cells. The order of the used minigenes (SMN1, SMN2, RPGR wt, RPGR −2C and RPGR −6C) is indicated in the figure. The minigene mRNA products are indicated on the left. Quantification from three independent experiments as mean±s.d. percentage of exon inclusion is shown underneath the images. Significant differences between indicated samples are marked with asterisks: SMN2: control siRNA and Ipo9 siRNA ( P =0.03), RPGR −2C: control siRNA and Ipo9 siRNA ( P =0.02) and control siRNA and Exp6 siRNA ( P =0.04).

    Article Snippet: The antibody against the following protein was from Abnova: Ipo9 (PAB0154, 1:1000).

    Techniques: Confocal Microscopy, Bimolecular Fluorescence Complementation Assay, Transfection, Construct, Mini Gene Assay, Reverse Transcription Polymerase Chain Reaction, Control